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Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

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Title Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis
 
Creator Gao, Z Q
Meng, C X
Wang, G C
Ye, N H
 
Subject Porphyra yezoensis
Photosystem II
Filamentous sporophytes
Thylakoid membranes
Polypeptide composition
Sucrose density gradient ultracentrifugation
 
Description 244-249
Thylakoid membranes were isolated and purified from diploid filamentous sporophytes of Porphyra yezoensis Ueda
using sucrose density gradient ultracentrifugation (SDGUC). After thylakoid membranes were solubilized with SDS, the
phtosystem II (PSII) particles with high 2, 6-dichloroindophenol (DCIP) photoreduction activity were isolated by SDGUC.
The absorption and fluorescence spectra, DCIP photoreduction activity and oxygen evolution activity of the thylakoid
membranes and PSII particles were determined. The polypeptide composition of purified PSII particles was distinguished by
SDS-PAGE. Results showed that PSII particles of sporophytes differed from the gametophytes in spectral properties and
polypeptide composition. Apart from 55 kDa D1-D2 heterodimer, CP47, CP43, 33 kDa protein, D1, D2, cyt b559 and 12
kDa protein were identified from PSII particles from sporophytes; a new 102 kDa protein was also detected. However,
cyt c-550, 20 kDa, 14 kDa and 16 kDa proteins found in PSII particles from gametophytes were not detected in the sporophytes.
 
Date 2008-09-01T10:51:15Z
2008-09-01T10:51:15Z
2008-08
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/1939
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.45(4) [August 2008]