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Rapid detection of Fusarium oxysporum f. sp. ciceris from disease infested chickpea fields by loop-mediated isothermal amplification

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Relation http://oar.icrisat.org/10101/
http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/71229
 
Title Rapid detection of Fusarium oxysporum f. sp. ciceris from
disease infested chickpea fields by loop-mediated isothermal amplification
 
Creator Ghosh, R
Tarafdar, A
Sharma, M
 
Subject Pest Management
Chickpea
Plant Disease
 
Description Fusarium wilt (FW) in chickpea, caused by major fungal pathogen, Fusarium oxysporum f. sp. ciceris
(Foc) results in10-40% economic losses worldwide. In field condition, several soil borne pathogens produce similar
FW like symptoms in chickpea. Therefore, by visual symptoms, distinguish and diagnosis of FW disease is very
difficult in field. Hence, there is real need of an accurate, rapid and inexpensive diagnosis method for FW. In this study,
we developed hydroxynaphthol blue dye based a loop-mediated isothermal amplification (LAMP) assay targeting ef1á
gene sequence for rapid diagnosis of FW in chickpea. The reaction was optimized using minimum 10 fg of Focg DNA
at 63°C for 60 min. The characteristic sky blue colour and ladder like band in agarose gel of the final LAMP products
indicated the positive reaction. This LAMP based tool will be very helpful to rapid diagnosis and monitoring the
chickpea FW in the field.
 
Publisher Indian Phytopathological Society
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10101/1/2017%20INDIAN%20PHYTOPATHOLOGY.pdf
Ghosh, R and Tarafdar, A and Sharma, M (2016) Rapid detection of Fusarium oxysporum f. sp. ciceris from disease infested chickpea fields by loop-mediated isothermal amplification. Indian Phytopathology, 69 (4). pp. 47-50. ISSN 2248-9800