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Cloning and characterization of histone H3 and replacement histone H3.3 encoding genes of Manila clam (Ruditapes philippinarum)

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Title Cloning and characterization of histone H3 and replacement histone H3.3 encoding genes of Manila clam (Ruditapes philippinarum)
 
Creator Lu, Sumin
Bao, Zhenmin
Wang, Xiaolong
Hu, Jingjie
Fang, Jianguang
 
Subject Histone
Manila clam (Ruditapes philippinarum)
Replication dependent histone
Replication independent histone
 
Description 456-464
A full-length cDNA (rp-h3.3) encoding a replacement histone H3.3 had been identified in Manila clam (Ruditapes philippinarum). The cDNA consisted of 975 bp, including a ORF 411 bp in length, a 3′ untranslated regions 490 bp in length, a poly (A) tail and two polyadenylation signals. In addition, the cDNA encoded a peptide with the S.//.A.IG amino acid motif, which was identical to the replacement histone H3.3 variant. rpg-h3.3 and rp-h3s two sequences were obtained by amplifying the genomic DNA of R. philippinarum. The rpg-h3.3, which was the genomic DNA sequence of rp-h3.3, had one intron and the rp-h3s had no intron. It was predicted that h3s might be the h3l-like gene of R. philippinarum. Histone h3 (rp-h3) was also cloned from R. philippinarum genomic DNA. Therefore, the evolutionary origin between histone h3 and its replacement subtypes was analyzed. The study inclined to the viewpoint that h3l-like was the ancesteral gene of h3 and h3.3, which might be two different offsets from h3l-like. During histone evolution process, one copy of h3l-like genes became h3.3 by introns insertion and S-phase-coupled regulatory mechanisms lose, another copy became h3 by gene-duplication and accumulation of mutations in the S.//.A.IG motif.
 
Date 2008-11-05T04:38:14Z
2008-11-05T04:38:14Z
2008-10
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/2364
 
Language en_US
 
Publisher CSIR
 
Source IJBT Vol.7(4) [October 2008]