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Genetic variability among sheep breeds by random amplified polymorphic DNA-PCR

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Title Genetic variability among sheep breeds by random amplified polymorphic DNA-PCR
 
Creator Kumar, S
Kolte, A P
Yadav, B R
Kumar, Sushil
Arora, A L
Singh, V K
 
Subject Dendrogram
Genetic distance
Genetic identity
RAPD-PCR
Sheep breeds
 
Description 482-486
Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was employed to assess the genetic variability and phylogenetic relationship among six breeds of sheep, viz., Malpura, Kheri, Chokla, Garole and two crossbreds Avikalin and Bharat Merino. Twenty-four individuals from each breed/crossbred were selected randomly. Initially, 40 primers were screened, of which 16 were found polymorphic and utilized for estimation of genetic variability and phylogenetic relationship among the breeds. The genetic distance was found highest between Malpura and Garole (D=0.1428), and the lowest (D=0.0612) between Avikalin and Chokla. However, the genetic identity was observed highest (I = 0.9406) between Avikalin and Chokla and the lowest (I = 0.8669) between Malpura and Garole. The Kheri sheep was found close with Chokla (D=0.0741), followed by Malpura sheep (D=0.0902) based on genetic distance. The phylogenetic tree also showed that Avikalin and Chokla are more close, whereas Malpura and Garole are distant to each other. The present study suggested that RAPD-PCR can be used successfully for analyzing genetic variation and phylogenetic relationship among breeds of sheep.
 
Date 2008-11-05T04:43:33Z
2008-11-05T04:43:33Z
2008-10
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/2368
 
Language en_US
 
Publisher CSIR
 
Source IJBT Vol.7(4) [October 2008]