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PCR fingerprinting for identification and discrimination of plant-associated facultative methylobacteria

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Title PCR fingerprinting for identification and discrimination of plant-associated facultative methylobacteria
 
Creator Raja, P
Balachandar, D
Sundaram, S P
 
Subject ARDRA
BOX-PCR
ERIC-PCR
Methylobacterium
RAPD
RISA
 
Description 508-514
Pink-pigmented facultative methylotrophic bacteria (PPFMs) belonging to the genus Methylobacterium, often associated with phyllosphere and roots and symbiotically benefit the plant species. For identification and discrimination of common plant-associated Methylobacterium species, different PCR-based DNA fingerprinting techniques were evaluated along with metabolic divergence. Standard/type strains of 10 species of plant-associated Methylobacterium were fingerprinted by means of RAPD, ARDRA, RISA, BOX and ERIC markers. These species showed divergence in carbon-substrates utilization pattern. Among the above PCR techniques evaluated, RAPD, RISA and ERIC fingerprinting had high discriminatory power than ARDRA and BOX-PCR. Being simple, rapid and repeatable, RAPD, RISA and ERIC-PCR techniques could be used for species identification and diversity analysis of plant-associated PPFMs belonging to Methylobacterium.
 
Date 2008-11-05T04:18:43Z
2008-11-05T04:18:43Z
2008-10
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/2348
 
Language en_US
 
Publisher CSIR
 
Source IJBT Vol.7(4) [October 2008]