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Effect of wheat straw powder on enhancement of ligninolytic enzyme activity using Phanerochate chrysosporium

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Title Effect of wheat straw powder on enhancement of ligninolytic enzyme activity using Phanerochate chrysosporium
 
Creator Hossain, S Masud
Anantharaman, N
 
Subject Enhancement
Ligninolytic enzymes
Optimization
Wheat straw
White-rot fungus
Phanerochate chrysosporium
 
Description 502-507
A comparison made between the suspension culture of white-rot fungus, Phanerochate chrysosporium supplemented with wheat straw powder and control conditions (without wheat straw), revealed an enhancement in the ligninolytic enzymes activity in liquid shake flask in the former. Addition of 2% w/v of wheat straw powder improved greater stability to the lignin peroxidase (Li P) and manganese peroxidase (Mn P) enzymes when compared with the control. At optimum pH (4.5) and optimum temperature (40ºC), maximum enzymatic activity was observed. The optimum shaking speed was found to be 60 rpm. The maximum activity of 538 and 512 μ mol/min mL was obtained for Li P and Mn P with wheat straw powder by the white-rot fungus at optimum conditions, respectively. However, maximum activity of 239 and 215 μ mol/min mL, respectively was obtained for Li P and Mn P at similar conditions in the control.
 
Date 2008-11-05T04:28:24Z
2008-11-05T04:28:24Z
2008-10
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/2354
 
Language en_US
 
Publisher CSIR
 
Source IJBT Vol.7(4) [October 2008]