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Cloning of pediocin PA-1 and its immunity genes from Pediococcus acidilactici K7 using pAMJ shuttle vector into Lactococcus lactis MG1363

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Title Cloning of pediocin PA-1 and its immunity genes from Pediococcus acidilactici K7 using pAMJ shuttle vector into Lactococcus lactis MG1363
 
Creator Srikanth, Anusha
Halami, Prakash M
 
Subject Pediococcus acidilactici
Pediocin PA-1
Electroporation
P170 expression system
Lactococcus lactis
 
Description 550-553
The matured pediocin PA-1 encoding gene, pedA and its immunity counterpart, pedB from Pediococcus acidilactici K7 were cloned by PCR technique and ligated into the E. coli-lactic shuttle, protein expression vector pAMJ2008. The recombinant pAMJAB was constructed and sequenced to confirm the intactness of reading frame of the deduced pediocin PA-1 fusion protein. Lactococcus lactis MG1363 was electroporated with the plasmids and the transformation efficiency of ~ 2-3  10³ g⁻¹ was observed. This was further confirmed by plasmid DNA isolation and analysis.
 
Date 2008-11-05T04:29:31Z
2008-11-05T04:29:31Z
2008-10
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/2355
 
Language en_US
 
Publisher CSIR
 
Source IJBT Vol.7(4) [October 2008]