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Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

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Title Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells
 
Creator Priya, S
Sudhakaran, P R
 
Subject Hepatic stellate cells
Apoptosis
Curcumin
Hepatic fibrosis
Retinol
 
Description 317-325
Hepatic stellate cells (HSCs) undergo activation and transdifferentiation to myofibroblast like cells in liver injury,
leading to liver fibrosis. During recovery from injury, activated HSCs may either revert back to quiescent state or undergo
apoptosis or both. In the present study, we have examined whether recovery from hepatic injury involves apoptosis of
activated HSCs and tested whether curcumin (the yellow pigment from Curcuma longa Linn.) promotes recovery from
hepatic injury by inducing apoptosis of these cells. Hepatic injury was induced by CClâ‚„ and apoptosis was studied in HSCs
isolated from liver by MTT assay, DNA fragmentation, and DAPI and annexin staining. Hepatic recovery was assessed by
measuring hepatic marker activities, such as serum GOT, GPT and protein. Hepatic recovery occurred within 4 weeks after
inducing injury in untreated control, whereas curcumin treatment caused hepatic recovery within 2 weeks, as evidenced by
the reduction of hepatic marker activities to near normal levels. HSCs isolated from liver of animals treated with curcumin
showed maximum apoptotic marker activities in 2nd week, whereas in HSCs from untreated control recovering from injury,
maximum apoptosis was observed in 4th week. Induction of apoptosis in vivo during hepatic recovery was also suggested by
increase in caspase-3 activity. Treatment of isolated HSCs in culture with curcumin caused apoptosis during later stages
confirming that curcumin induced apoptosis of activated HSCs and not in unactivated quiescent HSCs. These results
suggested that hepatoprotective effect of curcumin causing recovery from injury involved apoptosis of activated HSCs.
 
Date 2008-11-06T04:10:28Z
2008-11-06T04:10:28Z
2008-10
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/2373
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.45(5) [October 2008]