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Enzymatic characteristics of quercetinases from some indigenous Aspergillus flavus strains

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Title Enzymatic characteristics of quercetinases from some indigenous Aspergillus flavus strains
 
Creator Yadav, R S S
Yadav, K D S
 
Subject Quercetinase
Aspergillus flavus
Dioxygenase
Copper enzyme
 
Description 345-349
Five indigenous Aspergillus flavus strains MTCC-2206,
1884, 1883, 1783 and 2456 were screened for the secretion of
quercetinase. Fungal strains MTCC-2206, 1884, 1883, and 1783
were found to secrete the quercetinase in the range of 0.24-0.36
enzyme unit/mL of the culture medium, while MTCC-2456
secreted only 0.04 enzyme unit/mL. The enzymatic characteristics
of quercetinase were determined. The Km values using quercetin
as the substrate were 12.5 μM, 14.0 μM, 12.5 μM and 13.0 μM for
the quercetinase produced by MTCC-2206, 1884, 1883 and 1783,
respectively. The pH optima for the above enzymes were 6.5, 6.5,
6.0 and 6.0 and temperature optima were 45, 40, 45 and 50ºC,
respectively. The partial purification from only one strain MTCC-
2206 was achieved (nearly 3-fold purification).
 
Date 2008-11-06T04:14:05Z
2008-11-06T04:14:05Z
2008-10
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/2377
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.45(5) [October 2008]