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Kinetics of α-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants

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Title Kinetics of α-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants
 
Creator Ghosh, Kallol K
Verma, Santosh Kumar
 
Subject α-Chymotrypsin
4-Nitrophenyl acetate
Hydrolysis
Micellar enzymology
Alkyldimethyl ethanolammonium bromide
Surfactants
 
Description 350-353
The kinetics of α-chymotrypsin (α-CT) catalyzed hydrolysis of
4-nitrophenyl acetate has been studied in aqueous solution of
alkyldimethylethanolammonium bromide (cetyl, dodecyl, decyl)
surfactants at concentrations below and above their critical
micelle concentration. From Michaelis-Menten kinetics, the
catalytic rate constant kcat and the Michaelis constant KM have
been determined. The bell-shaped profiles of α-CT activity with
increasing surfactant concentrations indicate the interaction
between the micelle-bound enzyme and substrate.
 
Date 2008-11-06T04:14:55Z
2008-11-06T04:14:55Z
2008-10
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/2378
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.45(5) [October 2008]