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Application of Box-Benhken experimental design for optimization of laccase production by Coriolus versicolor MTCC138 in solid-state fermentation

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Title Application of Box-Benhken experimental design for optimization of laccase production by Coriolus versicolor MTCC138 in solid-state fermentation
 
Creator Mishra, Abha
Kumar, Sunil
Kumar, Sudhir
 
Subject Coriolus versicolor
Cyanobacterial biomass
Laccase
Response surface methodology
Solid-state fermentation
 
Description 1098-1107
Ligninocellulosic waste and cyanobacterial bloom was used for production of laccase by Coriolus versicolor MTCC138
using Box- Benhken design method. Empirical model developed through RSM (Response surface methodology) in terms of
effective operational factors of medium pH, temperature, moisture content, inducers, groundnut shell and cyanobacterial
biomass was found adequate to describe production of laccase by C. versicolor MTCC138 in solid state fermentation. Correlation
coefficient (0.9758) for laccase production indicated a good agreement between experimental and predicted values. Optimal
physico-chemical factors for laccase production were found to be: cyanobacterial biomass, 2.55 g; pH, 4.94; temperature,
29.96 C; moisture content, 70.96 % and CuSO₄, 1.58 mM. Due to high potentiality of laccase, C. versicolor can be exploited
industrially for developing a novel technology using inexpensive renewable resources. Box-Benhken approach appeared potential
in process application.
 
Date 2008-12-05T06:06:11Z
2008-12-05T06:06:11Z
2008-12
 
Type Article
 
Identifier 0022-4456
http://hdl.handle.net/123456789/2536
 
Language en_US
 
Publisher CSIR
 
Source JSIR Vol.67(12) [December 2008]