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Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design

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Title Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design
 
Creator Ellouze, Olfa
Fattouch, Sami
Mestiri, Faouzi
Aniba, Mohamed Radhouen
Marzouki, Mohamed Nejib
 
Subject Xylanase production
Sclerotinia sclerotiorum S2
Factorial design
Wheat bran
Culture aeration
Phosphate anion effect
 
Description 404-409
The improvement of xylanase production by Sclerotinia sclerotiorum S2 using a liquid fermentation culture was investigated. The optimized process was divided into three basic steps: (i) evaluating xylanase inducers using different agricultural residues such as wheat bran, oat bran, orange peel and barley bran at 1% final concentration, and also filter paper. Among these, wheat bran showed the maximum activity (2.5 U/ml) at 12 days post-inoculation; (ii) for optimization, we determined the optimal concentration of inducer, the effect of phosphate anion (K₂HPO₄/KH₂PO₄) and culture aeration using a rotary shaker at 100 and 180 rpm. The optimal conditions for these three factors were determined in an experimental panel using factorial data, in which a mathematical model (Minitab software) was fitted; (iii) The optimized culture medium containing a high level of wheat bran (3%) without K₂HPO₄/KH₂PO₄ and submitted to a high agitation (180 rpm/min) increased the xylanase production from 2.5 U/ml to 4 U/ml (1.6-fold).
 
Date 2008-12-31T06:11:54Z
2008-12-31T06:11:54Z
2008-12
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/2708
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.45(6) [December 2008]