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Stability-indicating HPLC method for determination of vitamins B₁, B₂, B₃ and B₆ in pharmaceutical liquid dosage form

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Title Stability-indicating HPLC method for determination of vitamins B₁, B₂, B₃ and B₆ in pharmaceutical liquid dosage form
 
Creator Thomas, Shino
Kumar, Rakesh
Sharma, Ashutosh
Issarani, Roshan
Nagori, Badri Prakash
 
Subject Vitamin B₁, B₂, B₃ and B₆
HPLC
Stability indicating
Validation
 
Description 598-603
A simple, selective, precise and stability-indicating high-performance liquid chromatographic (HPLC) method for determination of vitamins B₁, B₂, B₃ and B₆ in pharmaceutical liquid dosage form was developed and validated. The chromatographic conditions comprised a reversed-phase C₁₈ column (250 ´ 4.6 mm), 5 μ with a mobile phase consisting of a mixture of solution (0.015 M 1-hexane sulphonic acid sodium salt, pH 3.0 ± 0.05) and methanol in gradient elution. The flow rate was kept at 1.5 mL/min. and the detection was carried out at 280 nm. The retention times of vitamins B₃, B₂, B₆ and B₁ were 6.3, 15.1, 19.9 and 42.7 min. respectively. The linear regression analysis data for the calibration plots showed good linear relationship with coefficient of correlation values, r = 0.999 for vitamins B₁, B₂, B₃ and B₆ in the concentration ranges of 23.08-42.85, 9.61-17.84, 116.36-216.09 and 7.01-13.02 μg/mL respectively. The method was validated for precision, recovery and robustness. The vitamins undergo degradation under acidic, basic, peroxide, photochemical and thermal conditions. Statistical analysis proves that the method is reproducible and selective for the estimation of vitamins under study. As the method could effectively estimate the vitamins in presence of their degradation products, it can be employed as a stability-indicating method.
 
Date 2009-01-28T07:08:25Z
2009-01-28T07:08:25Z
2008-11
 
Type Article
 
Identifier 0971–457X
http://hdl.handle.net/123456789/2868
 
Language en_US
 
Publisher CSIR
 
Source IJCT Vol.15(6) [November 2008]