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Characterization of embryonic stem cells: A special focus on farm animals

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Title Characterization of embryonic stem cells: A special focus on farm animals
 
Creator Kumar, D
Anand, T
Singh, M K
Chauhan, M S
Manik, R S
 
Subject Embryonic stem cells
Characterization
Differentiation
Karyotyping
Epigenetics
Farm animals
 
Description 23-32
Embryonic stem (ES) cells are derived from the inner cell mass of blastocysts. They grow indefinitely while maintaining the pluripotency in the presence of specific growth factors such as leukemia inhibitory factor. The molecular mechanisms for self-renewal of pluripotent cells and the role of various growth factors involved in self-renewal as well as differentiation are being deciphered. ES cells, in their undifferentiated state, are characterized by a distinct morphology and by the presence of a set of markers classified into intracellular and extracellular types. Expression of specific markers (surface and transcription based) is an important criterion for pluripotent or undifferentiated state of cells. The expression of these markers is found to be exclusive to a particular species. A thorough understanding of the expression of these markers and of factors or conditions for the long-term culture of ES cells, without compromising their pluripotency and a stable genetic make-up is very important for the production and maintenance of ES cells from different species of farm animals. In this review, we present an overview of characterization of embryonic stem cells in farm animals.
 
Date 2009-02-04T06:52:00Z
2009-02-04T06:52:00Z
2009-01
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/2948
 
Language en_US
 
Publisher CSIR
 
Source IJBT Vol.8(1) [January 2009]