Record Details


Field	Value

Title	Standardization of different parameters for ‘particle gun’ mediated genetic transformation of sugarcane (Saccharum officinarum L.)

Creator	Kaur, Ajinder Gill, M S Gill, Raman Gosal, S S

Subject	embryogenic calli GUS assay ‘particle gun’ bombardment sugarcane transformation

Description	31-34 Three different parameters, viz. quantity of DNA, size of target tissue and distance between microcarrier launch assembly and target tissue (firing distance), for ‘particle gun’ (Bio-Rad) mediated transformation were optimized using embryogenic calli of sugarcane (target tissue), gusA (reporter gene) and hpt (hygromycin resistance gene). Of 12, 18 and 24 µL of DNA (gusA and hpt) suspension used per two bombardments, highest (33.33%) GUS expression was observed with 18 µL of suspension carrying 1.88 µg DNA. Bombardments made into 2-5 mm calli exhibited 55.55% GUS expression, while no expression was obtained in 7.5-12 mm calli after 7 h of their incubation in X-gluc. Out of different firing distances, viz. 2.5, 5.0, 7.5 and 10.0 cm, maximum intensity of GUS expression was recorded when calli were bombarded at a distance of 5.0 cm from the microcarrier launch assembly in the ‘particle gun’.

Date	2009-02-10T04:05:03Z 2009-02-10T04:05:03Z 2007-01

Type	Article

Identifier	0972-5849 http://hdl.handle.net/123456789/3009

Language	en_US

Relation	Int. Cl.⁸ A01H5/00; C12N15/9

Publisher	CSIR

Source	IJBT Vol.6(1) [January 2007]

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