Record Details

Plantlet regeneration of Pinus kesiya Royle ex Gord. from mature embryos

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Title Plantlet regeneration of Pinus kesiya Royle ex Gord. from mature embryos
 
Creator Tandon, Pramod
Kumaria, Suman
Choudhury, Hiranjit
 
Subject growth characteristics
micropropagation
shoot buds induction
zygotic embryos
Pinus kesiya
 
Description 262-266
A high frequency (90.5%) of shoot bud induction was observed in mature zygotic embryos of Pinus kesiya Royle
ex Gord. that were pre-cultured in Quoirin and Lepoivre (LP) medium containing 23.15 μM Kn for 4 wk and then transferred to growth regulator-free medium. Multiplication and elongation of the shoot buds resulted in 1/2 LP medium containing 0.5 μM IBA and 4.5 μM BAP or Kn. Rooting was 72.3% in isolated shoots that were treated with
53.76 μMNAA for 24 h and then cultured on water-agar medium. Plantlets were hardened and successfully established on soil collected from pine forests with 70% survival. Biomass of the micropropagated plants was 3.2 times higher than seed-derived plants.
 
Date 2009-02-10T04:34:17Z
2009-02-10T04:34:17Z
2007-04
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/3018
 
Language en_US
 
Relation Int. Cl.⁸ A01H4/00, 7/00
 
Publisher CSIR
 
Source IJBT Vol.6(2) [April 2007]