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Molecular markers for screening salinity response in Sorghum

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Title Molecular markers for screening salinity response in Sorghum
 
Creator Rao, M V Subba
Kumari, P Kusuma
Manga, V
Mani, N Sarada
 
Subject RAPD
RFLP
salinity tolerance
Sorghum
 
Description 271-273
Genomic DNAs from two salinity tolerant (IS 23190-Zm 90 & IS 23217-Zm 138), one moderately tolerant (IS 3566) and one sensitive(IS 213253-Zm 321) accessions of Sorghum were subjected to RFLP and RAPD analyses. A combination of digestion with Dra I and probing with BADH 1 distinguished the sensitive from the tolerant and moderately tolerant accessions in the RFLP patterns. A primer having CGGCTAGGT sequence provided distinct RAPD pattern for the tolerants (presence of specific amplified products a, b & c), moderately tolerant (presence of only c) and sensitive (lacking a, b & c) genotypes.
 
Date 2009-02-10T06:39:48Z
2009-02-10T06:39:48Z
2007-04
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/3031
 
Language en_US
 
Relation Int. Cl.⁸ C12N 15/10
 
Publisher CSIR
 
Source IJBT Vol.6(2) [April 2007]