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Hapten synthesis, generation of polyclonal antibodies and development of ELISA for determination of thiamethoxam residues in food and environmental samples

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Title Hapten synthesis, generation of polyclonal antibodies and development of ELISA for determination of thiamethoxam residues in food and environmental samples
 
Creator Ramesh, Atmakuru
Thirugnanam, Perumal Elumalai
Balakrishnamurthy, Prakhya
 
Subject ELISA
residues
thaimethoxime
water and food samples
hapten
polyclonal antibodies
 
Description 365-371
An ELISA (enzyme-linked immunosorbent assay) method for the determination of thiamethoxam residues has been developed. The method involves the synthesis of hapten and generation of polyclonal antibodies in rabbit using the hapten-protein (bovine serum albumin) conjugate carrier. Recovery experiments show the accuracy of estimation of thiamethoxam residues that was within the linear working range of 1 to 60 ng mL⁻¹ with the r²=0.992. Influence of different parameters, like detergent concentration, solvent, pH, ionic strength, has been studied. Cross reactivity was tested using different analogs. The method tolerates the solvent methanol concentration up to 15%. However, other polar solvents influence negatively on the binding. The detergent Tween 20 has not made any impact on the absorbance. The optimized ELISA for thiamethoxam used 1-60 ng/well and an antiserum dilution between 10000 and 200000. Recoveries were above 98%. The method was successfully applied for the analysis of food and environmental samples.
 
Date 2009-02-10T07:45:01Z
2009-02-10T07:45:01Z
2007-07
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/3061
 
Language en_US
 
Relation Int. Cl.⁸ C07K19/00; G01N33/53
 
Publisher CSIR
 
Source IJBT Vol.6(3) [July 2007]