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A repeatable protocol for in vitro micropropagation of mulberry variety S₅₄

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Title A repeatable protocol for in vitro micropropagation of mulberry variety S₅₄
 
Creator Kavyashree, R
 
Subject apical bud
ex vitro rooting
micropropagation
multiple shoots
survival frequency
 
Description 385-388
Apical buds of mulberry (Morus indica L.) variety S₅₄ were cultured on LSBM supplemented with different concentrations of BAP and TIBA to study their in vitro micropropagation potential. It was found that LSBM fortified with 8.88 µM BAP in combination with 2 µM TIBA was the most suitable medium for initiation (94% response) and multiplication of shoots (10.6) when compared to other concentrations tried. The well developed, elongated, healthy multiple shoots were subjected to ex vitro rooting technique to obtain complete plantlets. The statistical analysis of the data revealed significant difference between and within the treatments with regard to multiple shoots and root formation. The well developed axenic plants with similar genetic makeup were successfully established in the field with 90% survival frequency through sequential hardening process.
 
Date 2009-02-10T06:50:46Z
2009-02-10T06:50:46Z
2007-07
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/3043
 
Language en_US
 
Relation Int. Cl.⁸ A01H4/00, 5/04
 
Publisher CSIR
 
Source IJBT Vol.6(3) [July 2007]