Record Details

Isolation and molecular characterization of β-tubulin gene from Arthrobotrys musiformis — A nematode trapping fungus

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Isolation and molecular characterization of β-tubulin gene from Arthrobotrys musiformis — A nematode trapping fungus
 
Creator Chauhan, J B
Priya, R Swetha
Jarullah, Bushra
Subramanian, R B
 
Subject Arthrobotrys musiformis
nemataphagous fungus
β-tubulin
PCR
pGEM-T-easy vector
benzimidazole
 
Description 404-406
A new isolate of Arthrobotrys musiformis, a naturally occurring predacious hyphomycete, has been found to be effective against Haemonchus contortus, a roundworm, which seriously affects milk and meat production in ruminants. However, further studies on the suitability of this fungus for use in integrated pest management (IPM) showed that it is highly susceptible to benzimidazole derivatives, which are the most common antihelmintics currently used for deworming cattle. β-tubulin plays a major role in conferring resistance to benzimidazols. The β-tubulin gene of A. musiformis was isolated from genomic DNA through PCR and sequenced. BLAST analysis revealed 95% homology with β-tubulin gene of A. oligospora followed by other filamentous fungi. The isolated gene lacks mutation, which confers susceptibility to benzimidazols in the gene sequence of
A. musiformis.
 
Date 2009-02-10T07:06:09Z
2009-02-10T07:06:09Z
2007-07
 
Type Article
 
Identifier 0972-5849
http://hdl.handle.net/123456789/3045
 
Language en_US
 
Relation Int. Cl.⁸ C12N15/10
 
Publisher CSIR
 
Source IJBT Vol.6(3) [July 2007]