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Antagonistic effect of polyamines on ABA-induced suppression of mitosis in Allium cepa L.

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Title Antagonistic effect of polyamines on ABA-induced suppression of mitosis in Allium cepa L.
 
Creator Mahajan, Arpana
Sharma, Shashi
 
Subject ABA
Allium cepa
Catalase
Mitotic Index
Putrescine
Spermidine
Spermine
 
Description 136-139
Effect of abscisic acid (ABA) and polyamines (PAs) [putrescine (Put), spermidine (Spd) and spermine (Spm)] on mitosis in root tips of A. cepa was studied. Treatment with ABA (0.1 to 100 μM) for 24 hr suppressed the mitosis, measured as mitotic index (MI), in a concentration-dependent manner with approx. 50% suppression at 10 μM of ABA. Treatment with different PAs (1 to 100 μM) had differential mitosis suppression effect. Spm was most inhibitory followed by Spd and Put, respectively. The higher concentrations of PAs (1 mM Put; 0.1 and 1 mM Spd or Spm) caused cell distortion. Remarkably, a 24 hr pretreatment of root tips with PAs prior to ABA (100 μM) treatment resulted in a general concentration-dependent reversal of ABA-induced suppression of MI. Catalase (CAT) activity in the root tips, an indicator of redox metabolism, increased due to ABA treatment in a concentration-dependent manner, remained unaltered in response to Put and declined due to Spd and Spm (≥0.1 mM). However, all PAs, irrespective of their individual effects, generally antagonized the ABA-dependent increase in CAT activity. Data indicate the possibility of ABA-PA interaction in the regulation of mitosis.
 
Date 2009-02-11T08:51:14Z
2009-02-11T08:51:14Z
2009-02
 
Type Article
 
Identifier 0019-5189
http://hdl.handle.net/123456789/3076
 
Language en_US
 
Publisher CSIR
 
Source IJEB Vol.47(2) [February 2009]