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High frequency plantlet regeneration from rhizomatous buds in Mantisia spathulata Schult. and Mantisia wengeri Fischer and analysis of genetic uniformity using RAPD markers

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Title High frequency plantlet regeneration from rhizomatous buds in Mantisia spathulata Schult. and Mantisia wengeri Fischer and analysis of genetic uniformity using RAPD markers
 
Creator Bhowmik, Sudipta Shekhar D
Kumaria, Suman
Rao, S R
Tandon, Pramod
 
Subject Mantisia spathulata
Mantisia wengeri
Plant regeneration
RAPD analysis
 
Description 140-146
A protocol has been devised for enhanced in vitro regeneration of critically endangered Mantisia spathulata Schult. and Mantisia wengeri Fischer. Highest Bud Forming Capacity (BFC) of 6.10±0.55 with an average of 19.93±3.19 roots was obtained for M. spathulata within 5-6 weeks in Murashige and Skoogs (MS) medium supplemented with a combination of 10.0 μM of N⁶-benzyladenine (BA) and 2.5μM of ⍺-naphtalene acetic acid (NAA). For M. wengeri, BFC of 7.82±0.73 and 20.86±1.65 roots was achieved in MS media supplemented with a combination of 5.0μM BA and 2.5μM of NAA RAPD markers were used to evaluate the genetic stability of in vitro raised hardened plantlets. Similarity coefficient among the regenerated plants ranged between 0.85-0.98 for M. spathulata and 0.83-0.98 for M. wengeri. Maximum of 88 and 90% genetic similarity were obtained between in vitro raised hardened plantlets and mother stock of M. spathulata and M. wengeri, respectively through RAPD analysis. The hardened plantlets after RAPD analysis on being transferred to soil of experimental garden showed no marked phenotypic variations in vegetative or floral characteristics.
 
Date 2009-02-11T09:09:41Z
2009-02-11T09:09:41Z
2009-02
 
Type Article
 
Identifier 0019-5189
http://hdl.handle.net/123456789/3077
 
Language en_US
 
Publisher CSIR
 
Source IJEB Vol.47(2) [February 2009]