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Characterization of thermo-tolerant and acid/alkali tolerant β-glucosidase from bacterial isolate M+

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Title Characterization of thermo-tolerant and acid/alkali tolerant β-glucosidase from bacterial isolate M+
 
Creator Bajaj, Bijender K
Pangotra, Himani
Wani, Masood A
Sharma, Ajay
Sharma, Priyanka
 
Subject Acid/alkali tolerant
Characterization
β-Glucosidase
Purification
Thermo-tolerant
 
Description 242-247
This study presents isolation and characterization of β-glucosidase (BG) from a bacterial isolate. Enzyme showed maximum activity at 50°C (2200 IU/l), however, relatively good activity (1600-1900 IU/l) was maintained even at higher temperature (60-80°C). Enzyme was thoroughly stable at 50-80°C for 30 min to 1 h and retained activity (75-100%), indicating its substantial thermo-tolerant nature. Maximum activity of BG was found at pH 6 (2200 IU/l), but significantly good activity was also observed at pH 5 (1800 IU/l), 7 (2000 IU/l), 8 (1900 IU/l) and 9 (1500 IU/l). Pb²⁺ caused considerable increase in enzyme activity (47%), while Zn²⁺ caused a little reduction (7%). SDS-PAGE showed a single band of BG (mol wt, 24 kDa).
 
Date 2009-02-18T10:25:14Z
2009-02-18T10:25:14Z
2009-03
 
Type Article
 
Identifier 0022-4456
http://hdl.handle.net/123456789/3160
 
Language en_US
 
Publisher CSIR
 
Source JSIR Vol.68(3) [March 2009]