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Kinetics and mechanism of reduction of ferricytochrome c by glutathione and L-cysteine: A comparative study
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View Archive Info| Field | Value | |
| Title |
Kinetics and mechanism of reduction of ferricytochrome c by glutathione and L-cysteine: A comparative study
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| Creator |
Subudhi, U
Chainy, G B N Mohanty, P |
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| Subject |
Ferricytochrome c
Glutathione L-cysteine Reduction |
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| Description |
37-40
The kinetics and mechanism of the reduction of ferricytochrome c [Cyt c(III)] by substrates namely glutathione (GSH) and L-cysteine (L-cys) have been investigated spectrophotometrically employing [substrate]T >> [Cyt c(III)]T. The reaction exhibits first order dependence in [substrate]T and [Cyt c(III)]T. The pseudo-first order rate constant increases with an increase in pH, indicating that the conjugate base form of the HCyt c(III) is a better oxidant than the parent HCyt c(III). The electron transfer rate constants between the oxidants and GSH for both the k₁ and k₂ paths are found to be greater than that with L-cysteine. Hence, GSH is a better reductant of Cyt c(III) as compared to L-cysteine. A suitable mechanism has been proposed on the basis of experimental findings. The deprotonation constant for HCyt c(III) and the second order rate constants of k₁ and k₂ paths for the present reaction at 25ºC have been determined. |
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| Date |
2009-02-24T05:00:40Z
2009-02-24T05:00:40Z 2006-02 |
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| Type |
Article
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| Identifier |
0301-1208
http://hdl.handle.net/123456789/3255 |
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| Language |
en_US
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| Relation |
C07K14/80
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| Publisher |
CSIR
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| Source |
IJBB Vol.43(1) [February 2006]
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