Induction, purification and characterization of an antibacterial peptide scolopendrin I from the venom of centipede Scolopendra subspinipes mutilans
NOPR - NISCAIR Online Periodicals Repository
View Archive InfoField | Value | |
Title |
Induction, purification and characterization of an antibacterial peptide scolopendrin I from the venom of centipede Scolopendra subspinipes mutilans
|
|
Creator |
Wenhua, Ren
Shuangquan, Zhang Daxiang, Song Kaiya, Zhou Guang, Yang |
|
Subject |
Centipede
Scolopendra subspinipes mutilans Antibacterial peptide Scolopendrin I Venom E. coli K12D31 Induction Hemolytic activity Agglutination activity |
|
Description |
88-93
The crude venom of the centipede Scolopendra subspinipes mutilans, injected with Escherichia coli K₁₂D₃₁ for 3-4 days showed broad-spectrum antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. It showed good antibacterial activity against E. coli K₁₂D₃₁ at different temperatures, pH, and ionic strengths. The crude venom was heated at 100°C for 30 min, centrifuged at 10,000 rpm for 30 min at 4°C and the supernatants were obtained, from which an antibacterial fraction having a molecular mass of 3000-5000 Da, was further separated by ultrafiltration. A homogeneous antibacterial peptide named scolopendrin I, having a molecular mass of 4,498 Da, was isolated using cation-exchange chromatography and two steps of reverse-phase high performance liquid chromatography (RP-HPLC). Scolopendrin I did not show any hemolytic and agglutination activities at the concentration below 30 μM. |
|
Date |
2009-02-25T04:01:00Z
2009-02-25T04:01:00Z 2006-04 |
|
Type |
Article
|
|
Identifier |
0301-1208
http://hdl.handle.net/123456789/3265 |
|
Language |
en_US
|
|
Publisher |
CSIR
|
|
Source |
IJBB Vol.43(2) [April 2006]
|
|