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Induction, purification and characterization of an antibacterial peptide scolopendrin I from the venom of centipede Scolopendra subspinipes mutilans

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Title Induction, purification and characterization of an antibacterial peptide scolopendrin I from the venom of centipede Scolopendra subspinipes mutilans
 
Creator Wenhua, Ren
Shuangquan, Zhang
Daxiang, Song
Kaiya, Zhou
Guang, Yang
 
Subject Centipede
Scolopendra subspinipes mutilans
Antibacterial peptide
Scolopendrin I
Venom
E. coli K12D31
Induction
Hemolytic activity
Agglutination activity
 
Description 88-93
The crude venom of the centipede Scolopendra subspinipes mutilans, injected with Escherichia coli K₁₂D₃₁ for 3-4 days showed broad-spectrum antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. It showed good antibacterial activity against E. coli K₁₂D₃₁ at different temperatures, pH, and ionic strengths. The crude venom was heated at 100°C for 30 min, centrifuged at 10,000 rpm for 30 min at 4°C and the supernatants were obtained, from which an antibacterial fraction having a molecular mass of 3000-5000 Da, was further separated by ultrafiltration. A homogeneous antibacterial peptide named scolopendrin I, having a molecular mass of 4,498 Da, was isolated using cation-exchange chromatography and two steps of reverse-phase high performance liquid chromatography (RP-HPLC). Scolopendrin I did not show any hemolytic and agglutination activities at the concentration below 30 μM.
 
Date 2009-02-25T04:01:00Z
2009-02-25T04:01:00Z
2006-04
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3265
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.43(2) [April 2006]