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Ficus cunia agglutinin for recognition of bacteria
NOPR - NISCAIR Online Periodicals Repository
View Archive Info| Field | Value | |
| Title |
Ficus cunia agglutinin for recognition of bacteria
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| Creator |
Adhya, M
Singha, B Chatterjee, B P |
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| Subject |
Ficus cunia
Agglutinin Bacteria ELISA |
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| Description |
94-97
Interaction of bacteria with lectin using anti-lectin antibody by ELISA is an established method. In the present study, we have devised a simple ELISA using a biotinylated lectin and antibiotin-HRP. Ficus cunia agglutinin (FCA), which has shown the specificity towards / anomers of GlcNAc and other –NAc containing sugars like LacNAc and GlcNAc β(1-4/6)GlcNAc, was used as a model lectin for the study of interaction with immobilized microorganisms on ELISA plate. The bacterial cells of E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis and Staphylococcus aureus showed binding with FCA and the degree of binding was dependent on the bacterial surface antigen. This method is considered a simple technique to study the lectin-bacteria interaction. |
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| Date |
2009-02-25T04:01:25Z
2009-02-25T04:01:25Z 2006-02 |
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| Type |
Article
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| Identifier |
0301-1208
http://hdl.handle.net/123456789/3266 |
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| Language |
en_US
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| Publisher |
CSIR
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| Source |
IJBB Vol.43(2) [April 2006]
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