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Isolation of stress responsive Psb A gene from rice (Oryza sativa L.) using differential display

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Title Isolation of stress responsive Psb A gene from rice (Oryza sativa L.) using differential display
 
Creator Tyagi, Aruna
Chandra, Arti
 
Subject Gene
Drought stress
Rice
Oryza sativa L.
 
Description 244-246
Differential display (DD) experiments were performed on drought-tolerant rice (Oryza sativa L.) genotype N22 to identify both upregulated and downregulated partial cDNAs with respect to moisture stress. DNA polymorphism was detected between drought-stressed and control leaf tissues on the DD gels. A partial cDNA showing differential expression, with respect to moisture stress was isolated from the gel. Northern blotting analysis was performed using this cDNA as a probe and it was observed that mRNA corresponding to this transcript was accumulated to high level in rice leaves under water deficit stress. At the DNA sequence level, the partial cDNA showed homology with psb A gene encoding for D1 protein.
 
Date 2009-02-27T06:00:33Z
2009-02-27T06:00:33Z
2006-08
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3286
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.43(4) [August 2006]