Isolation of stress responsive Psb A gene from rice (Oryza sativa L.) using differential display
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Title |
Isolation of stress responsive Psb A gene from rice (Oryza sativa L.) using differential display
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Creator |
Tyagi, Aruna
Chandra, Arti |
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Subject |
Gene
Drought stress Rice Oryza sativa L. |
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Description |
244-246
Differential display (DD) experiments were performed on drought-tolerant rice (Oryza sativa L.) genotype N22 to identify both upregulated and downregulated partial cDNAs with respect to moisture stress. DNA polymorphism was detected between drought-stressed and control leaf tissues on the DD gels. A partial cDNA showing differential expression, with respect to moisture stress was isolated from the gel. Northern blotting analysis was performed using this cDNA as a probe and it was observed that mRNA corresponding to this transcript was accumulated to high level in rice leaves under water deficit stress. At the DNA sequence level, the partial cDNA showed homology with psb A gene encoding for D1 protein. |
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Date |
2009-02-27T06:00:33Z
2009-02-27T06:00:33Z 2006-08 |
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Type |
Article
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Identifier |
0301-1208
http://hdl.handle.net/123456789/3286 |
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Language |
en_US
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Publisher |
CSIR
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Source |
IJBB Vol.43(4) [August 2006]
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