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Effect of Bacillus subtilis PB6, a natural probiotic on colon mucosal inflammation and plasma cytokines levels in inflammatory bowel disease

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Title Effect of Bacillus subtilis PB6, a natural probiotic on colon mucosal inflammation and plasma cytokines levels in inflammatory bowel disease
 
Creator Selvam, R
Maheswari, P
Kavitha, P
Ravichandran, M
Sas, Benedikt
Ramchand, C N
 
Subject Inflammatory bowel disease
Bacillus subtilis PB6
TNBS-induced colitis
Wistar rats
Cytokines
 
Description 79-85
The pathophysiology of inflammatory bowel disease (IBD) involves the production of diverse lipid mediators, namely eicosanoid, lysophospholipids, and platelet-activating factor, in which phospholipase A₂ (PLA₂) is the key enzyme. Thus, it has been postulated that control of lipid mediators production by inhibition of PLA₂ would be useful for the treatment of IBD. This hypothesis has been tested in the present study by examining the therapeutic effect of a novel natural probitic Bacillus subtilis PB6 (ATCC- PTA 6737). B. subtilis PB6 is found to secrete surfactins (cyclic lipopeptides) which have anti-bacterial potential. These surfactins inhibit PLA₂, a rate-limiting enzyme involved in the arachidonic acid associated inflammatory pathway and could downregulate the inflammatory response by regulating the eicosanoid and cytokine pathways. With this concept, an experimental animal trial has been conducted in a rat model of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. The oral administration of PB6 suppresses the colitis as measured by mortality rate, changes in the weight gain, colon morphology and the levels of plasma cytokines. The animals treated orally with PB6 at
1.5 × 10⁸ CFU/kg thrice daily from day 4 to 10 significantly improve gross pathology of the colon and regain the colon weight to normal (p< 0.05), compared to TNBS-induced positive control. The plasma levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6 and IFN-γ) are also significantly lowered (p
 
Date 2009-03-05T06:33:07Z
2009-03-05T06:33:07Z
2009-02
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3339
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(1) [February 2009]