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N and N+1 cap effects of Gly, Ala, Leu, Val, Pro and Aib in Boc-(D)Glu₁-Xxx₂-Yyy₃-Lys₄-NHMe, a 3₁₀ type protohelix endlocked by Boc-(D)Glu

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Title N and N+1 cap effects of Gly, Ala, Leu, Val, Pro and Aib in Boc-(D)Glu₁-Xxx₂-Yyy₃-Lys₄-NHMe, a 3₁₀ type protohelix endlocked by Boc-(D)Glu
 
Creator Bobade, V D
Gaikwad, N D
Mhaske, P C
 
Subject 3₁₀ Helix
II′ turn
Reverse turn
D amino acids
NMR studies
 
Description 386-396
Gly, Ala, Leu, Val, Pro and Aib are introduced as N cap (Xxx₂) or N+1 cap (Yyy₃) residues in Boc-(D)Glu₁-Xxx₂- Yyy₃-Lys₄-NHMe, a 3₁₀ like protohelix endcapped by Boc-(D)Glu. NOEs, NH temperature coefficients and ³JNHCα coupling constants establish that all variants, except Boc-(D)Glu₁-Ala₂-Pro₃-Lys₄-NHMe, 1e are distorted 3₁₀ type protohelices, while the response of Glu₁ NH- a reporter of Lys₄→Glu₁ salt bridge-to solvent, salt and temperature induced perturbations, reveals that the protohelix variants differ appreciably in the degree of ordering and overall stability. The capping position variations are thus shown to affect the protohelix stability in a manner that is strongly reminiscent of the presumed effects in helix nucleation.
 
Date 2009-03-25T04:01:04Z
2009-03-25T04:01:04Z
2009-03
 
Type Article
 
Identifier 0376-4699
http://hdl.handle.net/123456789/3462
 
Language en_US
 
Publisher CSIR
 
Source IJCB Vol.48B(3) [March 2009]