Isolation and characterization of two N-acetyl-D-lactosamine specific lectins from tubers of Arisaema intermedium Blume and A. wallichianum Hook f
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Title |
Isolation and characterization of two N-acetyl-D-lactosamine specific lectins from tubers of Arisaema intermedium Blume and A. wallichianum Hook f
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Creator |
Kaur, Manpreet
Singh, Jatinder Kamboj, Sukhdev Singh Singh, Jagmohan Kaur, Amandeep Sood, S K Saxena, A K |
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Subject |
Araceae
Arisaema tuber N-acetyl-D-lactosamine asialofetuin lectin mitogenic antiproliferative effect human lymphocytes human cancer cell lines carbohydrate specificity denaturing agents |
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Description |
34-40
Two new lectins were purified from the tubers of Arisaema intermedium Blume and A. wallichianum Hook. f. (family: Araceae) by affinity chromatography on asialofetuin-linked amino activated silica beads. The bound lectins were eluted with 0.1 M glycine-HCl, pH 2.5. They gave a single band corresponding to subunit Mr 13.4 kDa in SDS-PAGE, pH 8.3. On gel filtration chromatography, the lectins showed a Mr of 51.2 kDa, suggesting a homotetrameric structure. Both the lectins gave a single peak on size exclusion HPLC and cation-exchange columns and a single band on PAGE, pH 4.5. However, like other monocot lectins, they gave multiple bands in isoelectric focusing and at PAGE 8.3. The lectins were inhibited by N-acetyl-D-lactosamine (LacNAc), a disaccharide and asialofetuin, a complex desialylated serum glycoprotein. They had no requirement for divalent metal ions i.e., Ca²⁺ and Mn²⁺ for their activity and were found to be mitogenic towards human lymphocytes. A. intermedium showed antiproliferative effect against various human cancer cell lines in vitro. |
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Date |
2009-03-30T06:50:37Z
2009-03-30T06:50:37Z 2005-02 |
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Type |
Article
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Identifier |
0301-1208
http://hdl.handle.net/123456789/3498 |
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Language |
en_US
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Relation |
C 07K 14/42
G 01N 33/53 |
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Publisher |
CSIR
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Source |
IJBB Vol.42(1) [February 2005]
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