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Role of a protein inhibitor isolated from human renal stone matrix in urolithiasis

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Title Role of a protein inhibitor isolated from human renal stone matrix in urolithiasis
 
Creator Aggarwal, S
Tandon, C
Forouzandeh, M
Singla, S K
Kiran, R
Jethi, R K
 
Subject Kidney
Calculi
Calcium oxalate
Organic matrix
Inhibitor
 
Description 113-117
The role of biomolecule(s) from renal stone matrix in urolithiasis was investigated. The ability of a particular fraction (>10 kDa fraction) isolated from the EDTA extract of powdered human renal stones to influence calcium oxalate monohydrate (COM) crystal growth was studied. The most potent inhibitor of COM crystal growth obtained from >10 kDa fraction was purified by various chromatographic techniques and SDS-PAGE, etc. and was found to have a molecular mass of 36 kDa. The urine and serum samples obtained from normal persons were found to be more potent in inhibiting the growth of COM crystals as compared to the kidney-stone patients. Polyclonal antibodies were raised against this inhibitor and were employed to determine the concentration of 36 kDa inhibitor in urine and serum samples of normal persons and kidney-stone patients.
 
Date 2009-03-30T07:24:13Z
2009-03-30T07:24:13Z
2005-04
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3509
 
Language en_US
 
Relation G01N33/00
 
Publisher CSIR
 
Source IJBB Vol.42(2) [April 2005]