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Modulation of spontaneous electrical activity of freshly isolated 3-day embryonic chick ventricle by cAMP and cGMP

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Title Modulation of spontaneous electrical activity of freshly isolated 3-day embryonic chick ventricle by cAMP and cGMP
 
Creator Prakash, P
Tripathi, O
 
Subject cAMP
cGMP
Action potential
Embryonic chick ventricle
Spontaneous electrical activity
Ca²⁺ channel
Chronotropic effect
 
Description 118-121
Effects of cyclic nucleotides 8-Bromo-cAMP and 8-Bromo-cGMP (membrane permeable analogs of cAMP and cGMP) were examined on action potential (AP) configuration and rate of spontaneous firing of the freshly isolated 3-day embryonic chick ventricle (ECV) to assess the role of L-type slow Ca²⁺ channels in upstroke of AP and spontaneous electrical activity (pacemaker potential). The 3-day ECV exhibited prominent automaticity and spontaneous APs characterized by maximum upstroke velocity (+Vmax), maximum diastolic potential (MDP), overshoot (Eov), AP duration at -20 mV (APD₂₀) and cycle length (CL) of 33.09±3.18 V/sec, -63.77±1.17 mV, 17.40±0.91 mV, 51.20±3.05 m sec and 795±150 m sec, respectively (n= 10 preparations). 8-Br-cAMP (1 mM) caused significant increase in Eov and APD₂₀ (37% and 56%, respectively, p
 
Date 2009-03-30T07:19:28Z
2009-03-30T07:19:28Z
2005-04
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3506
 
Language en_US
 
Relation C07H19/00, 19/20
 
Publisher CSIR
 
Source IJBB Vol.42(2) [April 2005]