Record Details

Stage-specific cytosolic protein kinase C-like activity in human malarial parasite Plasmodium falciparum

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Stage-specific cytosolic protein kinase C-like activity in human malarial parasite Plasmodium falciparum
 
Creator Sharma, Arun
Biswas, Sukla
 
Subject Malaria
Plasmodium falciparum
Protein kinase C
Cytosol
Membrane
Drug resistance
Chloroquine
Phorbol myristate acetate
Phosphatidyl serine
Schizont
Trophozoite
 
Description 145-151
Protein kinase C (PKC)-like activity was characterized in malarial parasite Plasmodium falciparum and its involvement in growth, maturation and differentiation functions, during the asexual stages (ring, trophozoite and schizont) of development was studied. PKC-like activity was found distributed in all the stages of the parasite maturation. The activity was predominantly cytosolic, however it was also present in the membrane fraction. The activation of cytosolic PKC required Ca²⁺, phosphatidyl serine (PS), and either diacylglycerol or phorbol myristate acetate (PMA). The 9-fold increase in the activity was observed in the presence of the co-factors (Ca²⁺, PS and PMA) in the late trophozoite stage, as compared to the ring stage. The activation of trophozoites with PMA resulted in redistribution of PKC-like activity from cytosol to membrane fractions. An antimalarial drug, chloroquine (CQ) inhibited directly the PKC-like activity in a dose-dependent manner (IC₅₀ of 45 nM) in trophozoites of chloroquine-sensitive CQ(S) strains, however, the activity remained unaltered in the chloroquine-resistant CQ(R) strains. Kinetic studies showed that the inhibition of cytosolic PKC-like activity by CQ was non-competitive with respect to ATP, histone and PS. The results suggest that the PKC-like activity is developmentally expressed during the parasitic survival and development.
 
Date 2009-03-30T07:42:05Z
2009-03-30T07:42:05Z
2005-06
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3517
 
Language en_US
 
Relation A 61 P 33/06
 
Publisher CSIR
 
Source IJBB Vol.42(3) [June 2005]