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Determination of serum glucose using co-immobilized glucose oxidase and peroxidase onto arylamine glass beads affixed on a plastic strip

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Title Determination of serum glucose using co-immobilized glucose oxidase and peroxidase onto arylamine glass beads affixed on a plastic strip
 
Creator Tank, Neelima
Suman
Pundir, C S
 
Subject Glucose determination
Glucose oxidase
Peroxidase
Arylamine glass
co-immobilization
Serum
Plastic strip
 
Description 391-394
Glucose oxidase (GOD) from Aspergillus niger and horserad-ish peroxidase (POD) were co-immobilized onto arylamine glass beads affixed on a plastic strip with a conjugation yield of 28.2 mg/g and 43% retention of their initial specific activity. The co-immobilized enzymes showed maximum activity at pH 7.5 when incubated at 37ºC for 15 min. A simple, specific and sensitive method for discrete analysis of the serum glucose was developed employing this strip. The minimum detection limit of the method was 5 mg/dl. Within and between assay coefficient of variations for the serum were
 
Date 2009-03-30T09:06:14Z
2009-03-30T09:06:14Z
2005-12
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3540
 
Language en_US
 
Relation C12N 11/00
 
Publisher CSIR
 
Source IJBB Vol. 42(6) [December 2005]