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Automated derivatization with o-phthalaldehyde for the estimation of amino acids in plasma using reversed-phase high performance liquid chromatography
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View Archive Info| Field | Value | |
| Title |
Automated derivatization with o-phthalaldehyde for the estimation of amino acids in plasma using reversed-phase high performance liquid chromatography
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| Creator |
Gnanou, J V
Srinivas, S K Kurpad, A V |
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| Subject |
o-Phthaladehyde derivatization
reversed-phase high performance liquid chromatography plasma amino acid analysis. |
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| Description |
322-325
A fully automated method for quantitative estimation of plasma amino acids using fluorescence detection of o-phthaladehyde/2-mercaptoethanol derivatives of the analytes and their separation by gradient elution reversed-phase HPLC has been described. The method is simple and the three-step gradient elution is suitable for routine analysis of a large number of biological samples due to clear resolution, high degree of precision, accuracy, cost-effectiveness and lack of interference from chemical contaminations. Using this method, 19 amino acids were completely resolved and the within-run coefficients of variation ranged from 2.53 to 10.7% with a mean variation of 5.68%. |
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| Date |
2009-04-02T10:42:21Z
2009-04-02T10:42:21Z 2004-12 |
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| Type |
Article
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| Identifier |
0301-1208
http://hdl.handle.net/123456789/3725 |
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| Language |
en_US
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| Relation |
G01 N 33
88 |
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| Publisher |
CSIR
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| Source |
IJBB Vol.41(6) [December 2004]
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