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Analysis of free nucleotide pools of mouse liver tissue by high-pressure liquid chromatography (HPLC)

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Title Analysis of free nucleotide pools of mouse liver tissue by high-pressure liquid chromatography (HPLC)
 
Creator Abadi, Reza Haji Hosseini Baghdad
 
Description 209-212
In this paper, analysis of free nucleotides from mouse liver tis-sue during different day times has been described. Perchloric acid extract of mouse liver tissue was neutralized with tri-N-octylamine in trichlorotriflouroethane and after removal of ClO-4, subjected to preliminary purification on a Cu2+-loaded column of Chelex 100. A high-pressure liquid chromatographic (HPLC) anion-exchange procedure used in the study gave a good resolu-tion of free nucleotides on a single column.
 
Date 2009-04-08T04:37:37Z
2009-04-08T04:37:37Z
2003-06
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3760
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.40(3) [June 2003]