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Nimesulide affects antioxidant status during acute lung inflammation in rats

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Title Nimesulide affects antioxidant status during acute lung inflammation in rats
 
Creator Sohi, Kiranjit K
Khanduja, Krishan L
 
Subject Nimesulide
antioxidant defense
superoxide anions
lipid peroxidation
 
Description 238-245
Antiradical activity of nimesulide, a commonly used COX-2 specific inhibitor, was estimated in vitro by 1, 1 diphenyl-2-picrylhydrazyl (DPPH) assay, nitroblue tetrazolium reduction assay and lipid peroxidation assay, respectively. The biochemistry of antioxidant functions of nimesulide was also investigated under control and inflammatory conditions, caused by intratracheal instillation of lipopolysaccharide (LPS). Pro-inflammatory conditions generally end up in oxidative insults, which have been suggested to be the cause of multiple organ failure in inflammation. A primary defense, constituted of antioxidant enzymes, against this oxidative damage has evolved in the body. In this study, male Wistar rats were orally administered with nimesulide (9 mg/kg/twice daily for 1 week), followed by intratracheal instillation with 2 μg of LPS and after 18 hr, antioxidant defense system and lipid peroxidation were measured in liver, lungs and kidneys. Nimesulide pretreatment was found to protect the tissue from enhanced levels of lipid peroxidation, and also stimulated the levels of glutathione-S-transferase (GST) in liver and glutathione reductase in kidneys. Surprisingly, nimesulide oral feeding also significantly suppressed superoxide dismutase (SOD) activity in all the three organs. Although, in our study, nimesulide proved to be an inducer of GST (a marker for chemoprevention) and a scavenger of superoxide anions at higher concentrations (> 250 μM), but the relevance of suppression of SOD enzyme activity, which may contribute to the drug’s toxic effects cannot be ignored. The work suggests that further long term studies are needed to confirm nimesulide as a safe drug.
 
Date 2009-04-13T09:58:55Z
2009-04-13T09:58:55Z
2003-08
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3797
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.40(4) [August 2003]