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Effect of oxidative stress on the spermatogenic process and hsp70 expressions in mice testes

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Title Effect of oxidative stress on the spermatogenic process and hsp70 expressions in mice testes
 
Creator Kaur, Parminder
Bansal, M P
 
Subject selenium
testis
hsp70
oxidative stress
 
Description 246-251
The effect of oxidative stress on the process of spermatogenesis in terms of hsp70 expression was studied. For creating different oxidative stressed mice, three selenium (Se) levels viz., deficient (group I), adequate (group II) and excess (group III) were fed for 8 weeks in a yeast-based diet. After completion of diet feeding, Se level was significantly decreased in group I and significantly increased in group III, as compared to group II. Glutathione peroxidase (GSH-Px) activity was significantly decreased in both liver and testis in group 1 animals; however, the activity was comparable in groups II and III. Significant increase in the testis glutathione-S-transferase (GST) activity was observed in group I. No change was seen in group III, when compared to group II. Histological analysis of testis revealed a significant decrease in the germ cell population in group I, as compared to group II, with a predominant effect on spermatid and mature sperm numbers. In group III, displacement of germ cell population was observed. ELISA assays for hsp70 level showed increase in group I as compared to group II, whereas no significant change was observed in group III, as compared to group II. Immuno-histochemical analysis revealed intense localization of hsp70 only in spermatid and sperm cells. The expression in groups II and III was homogeneous with slightly increased expression around lumen in group III. The data indicate that excessive oxidative stress in Se deficient group, affects the spermatogenesis process, especially affecting the mature sperm number which in turn leads to infertility.
 
Date 2009-04-13T09:59:05Z
2009-04-13T09:59:05Z
2003-08
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3798
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.40(4) [August 2003]