Record Details

Methods for estimating lipid peroxidation: An analysis of merits and demerits

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Methods for estimating lipid peroxidation: An analysis of merits and demerits
 
Creator Devasagayam, T P A
Boloor, K K
Ramasarma, T
 
Subject Lipid peroxidation
thiobarbituric acid reactive substances
malondialdehyde
polyunsaturated fatty acid
conjugated dienes
lipid hydroperoxides
estimation
 
Description 300-308
Among the cellular molecules, lipids that contain unsaturated fatty acids with more than one double bond are particularly susceptible to action of free radicals. The resulting reaction, known as lipid peroxidation, disrupts biological membranes and is thereby highly deleterious to their structure and function. Lipid peroxidation is being studied extensively in relation to disease, modulation by antioxidants and other contexts. A large number of by-products are formed during this process. These can be measured by different assays. The most common method used is the estimation of aldehydic products by their ability to react with thiobarbituric acid (TBA) that yield ‘thiobarbituric acid reactive substances’ (TBARS), which can be easily measured by spectrophotometry. Though this assay is sensitive and widely used, it is not specific and TBA reacts with a number of components present in biological samples. Hence caution should be used while employing this method. Wherever possible this assay should be combined with other assays for lipid peroxidation. Such methods are measurement of conjugated dienes, lipid hydroperoxides, individual aldehydes, exhaled gases like pentane, isoprostanes, etc. The modern methods also involve newer techniques involving HPLC, spectrofluorimetry, mass spectrometry, chemiluminescence etc. These and other modern methods are more specific and can be applied to measure lipid peroxidation. There are certain restraints, in terms of high cost and certain artifacts, and these should be considered while selecting the method for estimation. This review analyses the merits and demerits of various assays to measure lipid peroxidation.
 
Date 2009-04-13T10:10:46Z
2009-04-13T10:10:46Z
2003-10
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3805
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.40(5) [October 2003]