ICAR Research Data Repository for Knowledge Management
Changes in the activity of matrix metalloproteinases in regenerating rat liver after CCl<sub>4 </sub>-induced injury
NOPR - NISCAIR Online Periodicals Repository
View Archive Info| Field | Value | |
| Title |
Changes in the activity of matrix metalloproteinases in regenerating rat liver after CCl4 -induced injury
|
|
| Creator |
Jacob, Shiju
Sudhakaran, P R |
|
| Subject |
hepatic regeneration
matrix metalloproteinases (MMPs) extracelluar matrix (ECM) hepatocytes CCl4 |
|
| Description |
324-329
Molecular mechanisms involved in mediating alteration in cell matrix interaction have been examined by studying the changes in the activity of matrix metalloproteinases (MMPs) in CCl4 -induced regenerating liver, using zymography and ELISA. Activity of MMPs (72 kD, 92 kD and 130 kD gelatinases) in the rat liver increased progressively during acute injury till the 4th day and then decreased to near normal level after CCl4 administration (0.5 ml/100 g body wt.) on the 6th day. Hepatocyte lysate of injured liver on the 4th day showed significantly higher levels of MMP2 and MMP9 compared to the control. In the culture medium of hepatocytes, the levels of MMP2 and MMP9 increased progressively with the duration of culture, indicating that hepatocytes are the major source of these MMPs in regenerating liver. These results suggest an involvement of MMPs in matrix degradation and remodeling during regeneration after acute liver injury induced by CCl4 . |
|
| Date |
2009-04-13T10:20:25Z
2009-04-13T10:20:25Z 2003-10 |
|
| Type |
Article
|
|
| Identifier |
0301-1208
http://hdl.handle.net/123456789/3808 |
|
| Language |
en_US
|
|
| Publisher |
CSIR
|
|
| Source |
IJBB Vol.40(5) [October 2003]
|
|