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Activation of tobacco leaf polyphenol oxidase by sodium dodecyl sulfate

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Field Value
 
Title Activation of tobacco leaf polyphenol oxidase by sodium dodecyl sulfate
 
Creator Jiang, Hui
Shi, Chunhua
Xie, Yongshu
Xu, Xiaolong
Liu, Qingliang
 
Subject Sodium dodecyl sulfate (SDS)
polyphenol oxidase
tobacco leaf
pH effect
temperature effect
 
Description 350-353
The effect of sodium dodecyl sulfate (SDS) on purified tobacco leaf PPO (PPO II) was investigated at various pHs and temperatures. SDS increased the activity of PPO II due to the formation of SDS-PPO II complex, leading to conformational changes, thus making access to active center easier. The relationship between the activity and the molar ratio of SDS-PPO II to PPO II showed that the critical point reached a plateau of activity at the molar ratio of about 1.2. The pH had a significant effect on interaction between SDS and PPO II, as compared to PPO II. The optimum catalytic temperature of the complex rose by 10°C, suggesting that stabilization of the structure had been improved by the formation of complex.
 
Date 2009-04-13T10:32:10Z
2009-04-13T10:32:10Z
2003-10
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/3811
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.40(5) [October 2003]