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Detection of genetic variation in four <i>Ulva </i>species based on RAPD technique

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Title Detection of genetic variation in four Ulva species based on RAPD technique
 
Creator prasad, Umashankar
Deshmukhe, Geetanjali
Dwivedi, Alkesh
Singh, S. D.
 
Subject seaweed
Ulva
DNA isolation
genetic variation
RAPD
 
Description 52-56
DNA yield was obtained by using 2 protocols, CTAB (Cetyltrimethylammonium bromide) and modified Wattier et al; out of which the latter yielded more quantity of DNA (0.85 mg/g). Out of total amplified product 53% bands were shown monomorphic and remaining of the bands were polymorphic. The high frequency of polymorphic bands suggests that the isolates of Ulva represented in our collection have sufficient genetic diversity for conducting a valid heterosis experiment. The intra species genetic similarity (GS) value was found highest for U. lobata and lowest for U. fasciata. The interspecies GS value was found highest between U. fasciata/U. lactuca and lowest between U. lobata/U. reticulata. The large number of differences among isolates revealed by the RAPD technique indicate that it would be possible to establish a unique “fingerprint” for individual plants based on the combined results generated from a small collection of primers.
 
Date 2009-05-22T05:27:11Z
2009-05-22T05:27:11Z
2009-03
 
Type Article
 
Identifier 0379-5136
http://hdl.handle.net/123456789/4244
 
Language en_US
 
Publisher CSIR
 
Source IJMS Vol.38(1) [March 2009]