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<i style="">In vitro</i><b style=""> </b>sperm agglutination and spermicidal activity of protein isolated from a marine mollusc<b style=""> </b><i style="">Telescopium telescopium</i>(Gastropoda)

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Title In vitro sperm agglutination and spermicidal activity of protein isolated from a marine mollusc Telescopium telescopium(Gastropoda)
 
Creator Pakrashi, A
Datta, U
 
Description 93-97
The crude extract of spermatheca gland and its 50% ammonium sulphate fractionated protein (SF-50) were found to have sperm agglutinating and immobilizing effect on murine, ovine and human spermatozoa in vitro. The effects were dose-dependent. Human unwashed and washed sperm cells were found dead within 20 sec after application of 6 mg/ml and 5 mg /ml of SF-50 respectively. Hemolymph of the mollusc, however, showed much less agglutinating effect than that of SF-50. SF-50 caused coiling of spermatozoan tail, swelling of acrosome and neck region and extrusion of the sperm tail membrane. The protein is highly soluble in water and contains sialic acid binding lectin.
 
Date 2009-06-15T12:17:22Z
2009-06-15T12:17:22Z
2001-06
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/4607
 
Language en_US
 
Publisher CSIR
 
Source IJMS Vol.30(2) [June 2001]