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Antibacterial activity in the accessory nidamental gland extracts of the Indian squid, <i style="">Loligo duvauceli</i> Orbigny

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Title Antibacterial activity in the accessory nidamental gland extracts of the Indian squid, Loligo duvauceli Orbigny
 
Creator Gomathi
Nair, P.
R, J.
Sherief
M, P.
 
Subject squid
ccessory nidamental glands
antibacterial activity
chromatography
fatty acids
 
Description 100-104
Antibacterial
activity of accessory nidamental gland-butanol extracts from the Indian squid Loligo duvauceli in different stages of
maturity was studied. The activity was evaluated by disc-diffusion method using
five strains of bacteria. The extracts from ripe stage ANG showed antibacterial
activity against gram negative bacterial strains, Escherichia coli and Pseudomonas
aeruginosa, and gram positive bacteria, Staphylococcus
aureus. Immature and spent gland extracts did not show any antibacterial
activity. Spectrophotometric analysis of the ripe gland extract showed the
maximum absorbance at 498.5 nm. This infers the
presence of carotenoid pigments which impart the orange red colour to the ripe glands.
Thin layer chromatography of the ANG-butanol extract revealed the presence of
lipid components such as phospholipids, cholesterol, free fatty acids,
triglycerides, fatty acid esters, and cholesteryl esters. The total free fatty acid content was significantly higher in the
ripe ANG (16.0 ± 0.143 mg oleic acid/g tissue), in comparison to the immature
ANG (10.3 ± 0.114 mg oleic acid/g tissue). Gas chromatographic studies of
immature and ripe stages revealed the presence of a mixture of fatty acids. The
major unsaturated fatty acids content in the ripe stage was 1.973 mg/gm tissue,
whereas in immature stage it was only 0.251 mg/g tissue. Significantly higher
levels of unsaturated fatty acids in the ripe stage could be the factor
responsible for the antibacterial activity of the ANG-butanol extract.
 
Date 2010-04-28T06:31:44Z
2010-04-28T06:31:44Z
2010-03
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/8547
 
Language en_US
 
Publisher CSIR
 
Source IJMS Vol.39(1) [March 2010]