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Isolation and characterization of 37 kDa heparinase from the purple fluid of <i style="">Dolabella auricularia</i>

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Title Isolation and characterization of 37 kDa heparinase from the purple fluid of Dolabella auricularia
 
Creator Abirami, P.
Arumugam, M.
Ajithkumar, T.T.
Balasubramanian, T.
 
Subject Nudibranch heparinase
Dolabella auricularia
Purple fluid
DEAE-cellulose
 
Description 112-116
Present study was to
explore the heparinase and antiglycosidase activity of the purple fluid of Dolabella auricularia. Heparinase
activity was found as 56.42 IU/ml, further the activity was confirmed by APPT
test by the reduction of coagulation time with the addition of purple fluid of
about 7±0.2. This purple fluid was dialyzed and purified by anion exchange column
(DEAE-cellulose). Amount of protein in crude and purified sample was 2mg/ml and
7.6mg/ml. Apparent molecular weight of the purified heparinase was reveled by
SDS-PAGE as 37 kDa. Effects of various factors such as temperature and pH were
determined and recorded the maximum activity at 30ºC-50ºC and 7 respectively.
Antibacterial activity was also observed against four pathogens and activity
was observed maximum only in P.
aeruginosa, S. aureus. Antiglycosidase
activity exhibits about 120.9IU/ml which has been reduced when compared to that
standard. Present study suggests the purple fluid of D. auricularia has immense uses in pharmaceutical industries.
 
Date 2011-03-29T07:18:46Z
2011-03-29T07:18:46Z
2011-02
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/11378
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.40(1) [February 2011]