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Bioprospecting of marine derived actinomycetes with special reference to antimycobacterial activity

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Title Bioprospecting of marine derived actinomycetes with special reference to antimycobacterial activity
 
Creator Radhakrishnan, M
Balagurunathan, R
Selvakumar, N
Doble, Mukesh
Kumar, Vanaja
 
Subject Marine actinomycetes
Antimycobacterial activity
LRP assay
 
Description 407-410
Present
study consists the bioprospecting of marine actinomycetes with special
reference to antimycobacterial activity. Totally 49 actinomycetes were isolated
from sediment samples collected from mangrove area of Pitchavaram coastal areas
of Parangipettai and Andaman & Nicobar Islands and by using starch casein
agar medium. Based on the cultural and microscopic characteristics, 30 isolates
were identified as Streptomyces sp.
and 19 as rare actinomycetes including 11 Micromonospora
sp. Bioactive substance from all the isolates were produced by shake flask
fermentation method using yeast extract malt extract broth. Culture filtrate
and mycelial methanol extracts were tested against drug sensitive and drug
resistant clinical isolates of M.
tuberculosis and standard strain Mycobacterium
tuberculosis H37Rv by luciferase reporter phage (LRP) assay. Culture
filtrate and/or mycelial methanol extracts from 41 out of 49 actinomycetes
inhibited at least one of the M.
tuberculosis strains tested. Culture filtrates from actinomycetes strains
viz., R6 (Streptomyces sp), M1A6 (Micromonospora sp), M1A15 (Actinosynnemma sp), M1A18 (Micromonospora sp) and M1A23 (rare
actinomycete) showed more than 90% reduction in RLU by LRP assay. Isolation of
active compounds from these potent strains probably would lead to the discovery
of novel antiTB drugs.
 
Date 2011-07-19T04:48:22Z
2011-07-19T04:48:22Z
2011-06
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/12432
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.40(3) [June 2011]