Record Details

Isolation, characterization and growth response of biofilm forming bacteria <i style="">Bacillus pumilus</i> from the sea grass, <i>Halodule pinifolia</i> off Kanyakumari coast

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Isolation, characterization and growth response of biofilm forming bacteria Bacillus pumilus from the sea grass, Halodule pinifolia off Kanyakumari coast
 
Creator Merina, Medo
Lipton, A.P.
Wesley, S. Godwin
 
Subject Bacteria
Biofilm
Colony forming units
Sea grass extract
Growth response
 
Description 443-448
Bacteria from the biofilms of the blade of sea grass, Halodule pinifolia
occurring along the sea grass beds off Kanyakumari coast (Tamil Nadu) were
isolated. Bacteria identified included Pseudomonas,
Staphylococcus, E.coli and Bacillus
species. Predominant biofilm bacterium was characterized as Bacillus pumilus
(Accession No. HM006706) by standard biochemical and molecular methods. Sea
grass biofilm associated B. pumilus strain was aerobic, Gram positive,
rod-shaped, spore-forming motile bacteria. The 16s rDNA gene sequence of the
new isolate B. pumilus had shown maximum similarity (96%) with other B. pumilus strains PRE14, NHIC3, CT3. Growth
response due to interaction of B. pumilus in the presence of sea grass
extract was evaluated by inoculating in different concentration of sea grass
extract. Results indicated that more colony forming units (4.6×1011
CFUs) in Zobell marine broth supplemented with 20.0% of the water extract of Halodule
pinifolia.
 
Date 2011-07-19T04:50:13Z
2011-07-19T04:50:13Z
2011-06
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/12437
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.40(3) [June 2011]