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<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Isolation and Characterization of Photosystem<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt; font-family:SimSun;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:AR-SA" lang="EN-GB">Ⅱ<span style="font-size:11.0pt; mso-bidi-font-size:10.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:AR-SA" lang="EN-GB">Complex from Red Alga <i style="mso-bidi-font-style: normal">Porphyra haitanesis</i></span></span></span>

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Title Isolation and Characterization of PhotosystemⅡComplex from Red Alga Porphyra haitanesis
 
Creator Li, Ying Xia
Wang, Guang Ce
Niu, Jian Feng
Gao, Zheng Quan
Chen, Chang Sheng
 
Subject Porphyra haitanesis
Thylakoid membranes
Photosystem II]
 
Description 11-15
Thylakoid
membranes were isolated and purified from gametophyte of Porphyra haitanesis by
sucrose density gradient ultracentrifugation. After P. haitanesis gametophyte thylakoid membranes were solubilized with
sodium dodecyl sulfate (SDS), the highly pure and active photosystem (PS) II
complex was isolated and characterized by the room absorption and fluorescence
emission spectra, DCIP (2, 6-Dichloroindophenol) reduction and oxygen- evolving
rates. The PS II complex from P.
haitanesis had the
characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 nm) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Acquired PS Ⅱcomplex exhibited high oxygen evolution
(879 μmol O2. mg-1 Chl. h-1) in the present of
2.5 mM DCIP as an
artificial acceptor and was active in photoreduction of DCIP (2, 6-
dichloroindophenol) by DPC
(1, 5- diphenylcarbazide) at 104 ueq. mg-1.Chl a. h-1. Extrinsic proteins of P. haitanesis PS Ⅱconsisted of 33, 20, 16, 12 kD proteins
and cyt c-550 (15 kD), and were different with that of cyanobacteria and higher
plants.
 
Date 2013-02-19T12:50:48Z
2013-02-19T12:50:48Z
2013-02
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/15979
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.42(1) [February 2013]