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<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)</span>

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Title Comprehensive Extraction of Agar and R-Phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta)
 
Creator Niu, Jianfeng
Xu, Meiling
Wang, Guangce
Zhang, Kaiyue
Peng, Guang
 
Subject Gracilaria lemaneiformis
R-phycoerythrin
Agar
Expanded bed adsorption
Ion-exchange chromatography
 
Description 21-28
Water-soluble
proteins from Gracilaria lemaneiformis were extracted and further purification
for R-phycoerythrin (R-PE) was carried out.  Remaining cell mass was used for agar
extraction.  Approximately 21% of the
R-PE recovered from the crude extract and the yield of R-PE with a purification
ratio >3.2 were 0.36 mg g-1 fresh G. lemaneiformis. The yield of agar was 15.2%, with the gel
strength of 805±20 g
cm-2 in a 1%
gel at 30°C, which showed no significant difference from those obtained from
the directed agar extraction using the dried algae. Research in the experiments
also revealed that R-PE could purify from the waste hydration solution of the
dried alga, although its recovery and purity found to be lower than when it was
extracted from fresh algae.
 
Date 2013-02-19T12:55:36Z
2013-02-19T12:55:36Z
2013-02
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/15981
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.42(1) [February 2013]