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<span style="font-size:15.0pt;mso-bidi-font-size: 14.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN-GB">Phylogenetic Appraisal of the Drug Bearing Marine Sponge, <i style="mso-bidi-font-style:normal">Callyspongia subarmigera </i>(Ridley, 1884) from South India</span>

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Title Phylogenetic Appraisal of the Drug Bearing Marine Sponge, Callyspongia subarmigera (Ridley, 1884) from South India
 
Creator Aishwarya, M. S.
Lipton, A. P.
Sarika, A. R.
 
Subject Callyspongia subarmigera
Bioactivity
Methanol extract
PCR amplification
DNA sequencing
Phylogenetic tree
 
Description 139-145
Tropical Callyspongia subarmigera prevalent in the south-east coast of India
was studied for evaluating the bioactivity and phylogenetic resemblance with other
drug-bearing species. Antibacterial screening of the methanol extract of
C. subarmigera against clinical and
fish pathogens displayed their broad spectrum against Gram positive and Gram
negative pathogenic strains. Fish pathogenic bacteria Vibrio pelagius and Vibrio
fischeri and the clinical pathogen Pseudomonas
aeruginosa were highly susceptible to the methanol extract. Sponge extract
had no antifungal property against the two fungal strains tested viz., Aspergillus niger
and Candida albicans. Callyspongia subarmigera extract
indicated cytotoxic activity with an LC50 of 0.39% against Artermia nauplii in 24 h. PCR protocol
was carried out using a 16S rRNA primer and an 18S rRNA primer and the
annealing temperature was standardized at 55°C. BLAST analysis was conducted to
detect the resemblance of different species of Callyspongia genera of sponges. A phylogenetic tree was constructed

using neighbour joining method and the results revealed that C. subarmigera had the nearest homology
to that of
Haliclona cinerea, Haliclona oculata
(88%) and Callyspongia spp (88%)
possessing pharmacological relevance.
 
Date 2013-02-19T13:05:59Z
2013-02-19T13:05:59Z
2013-02
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/15997
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.42(1) [February 2013]